Sizing and Sorting of Single Dna Molecules by Microfluidic Molecular Combing Device
نویسندگان
چکیده
We report a microfluidic molecular combing device to trap, stretch, and separate DNA at a singlemolecule resolution. In order to align long DNA into a single direction on surface, we demonstrated molecular combing by dewetting DNA solution inside a microfluidic channel. It used a surface tensional force at the air-water interface of DNA solution translating on a fabricated pattern (zigzag line). When the speed of molecular translation and adsorption were balanced by pressure control, DNA was stretched along the direction of flow. By specifying the location of DNA attachment at the zigzag vertices, the device served as a molecular ruler for sizing and sorting of single DNA molecules.
منابع مشابه
A microfabricated device for sizing and sorting DNA molecules.
We have demonstrated a microfabricated single-molecule DNA sizing device. This device does not depend on mobility to measure molecule size, is 100 times faster than pulsed-field gel electrophoresis, and has a resolution that improves with increasing DNA length. It also requires a million times less sample than pulsed-field gel electrophoresis and has comparable resolution for large molecules. H...
متن کاملFluorescent Contrast agent Based on Graphene Quantum Dots Decorated Mesoporous Silica Nanoparticles for Detecting and Sorting Cancer Cells
Background and Objectives: The inability of classic fluorescence-activated cell sorting to single cancer cell sorting is one of the most significant drawbacks of this method. The sorting of cancer cells in microdroplets significantly influences our ability to analyze cancer cell proteins. Material and Methods: We adapted a developed microfluidic device as a 3D in vitro model to sorted MCF-7 c...
متن کاملFault Tolerant DNA Computing Based on Digital Microfluidic Biochips
Historically, DNA molecules have been known as the building blocks of life, later on in 1994, Leonard Adelman introduced a technique to utilize DNA molecules for a new kind of computation. According to the massive parallelism, huge storage capacity and the ability of using the DNA molecules inside the living tissue, this type of computation is applied in many application areas such as me...
متن کاملMicrofluidic assays for DNA manipulation based on a block copolymer immobilization strategy.
Methods to manipulate and visualize isolated DNA and oligonucleotide strands are important for investigation of their biophysics as well as their interactions with proteins. Herein, we report such a method by combining a block copolymer surface functionalization strategy with microfluidics. The copolymer poly(l-lysine-graft-polyethylene glycol) (PLL-g-PEG) coated one surface of the microfluidic...
متن کاملMicrofabricated Devices for Sizing DNA and Sorting Cells
We have microfabricated devices to size and sort microscopic biological objects, ranging from cells to single molecules of DNA. Sizing is accomplished by fluorescent excitation and detection. The devices are fabricated in a silicone elastomer using a replica method. Single molecules of DNA have been sized to 10% accuracy, and manipulation of E. Coli cells has been demonstrated.
متن کامل